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    SDS gels of amyloid fibre-chaperone complexes

    Cite as: Saibil, Helen and MONISTROL, Jim and Johnston, Erin (2023): SDS gels of amyloid fibre-chaperone complexes. Birkbeck College, University of London. doi: https://doi.org/10.18743/DATA.00000326

    Description

    Metadata in a spreadsheet
    Photos of the gels used for binding assays

    Collection Method

    Preparations of in vitro assembled alpha synuclein amyloid fibres were mixed with Hsc70, DNAJB1, Apg2 and ATP in varying combinations, then centrifuged to separate pellet and supernatant fractions, which were analysed by polyacrylamide gel electrophoresis to determine how much of the Hsc70 remained in the supernatant or pelleted with the fibres under different conditions.

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    Additional Metadata

    Data

    Metadata

    Dataset Title:

    SDS gels of amyloid fibre-chaperone complexes
    Creators:

    Saibil, Helen and MONISTROL, Jim and Johnston, Erin
    School/Department:

    Birkbeck Schools and Research Centres > School of Science > Biological Sciences
    Keywords:

    alpha synuclein amyloid, chaperones, Hsc70, DNAJB1
    Data collection method:

    Preparations of in vitro assembled alpha synuclein amyloid fibres were mixed with Hsc70, DNAJB1, Apg2 and ATP in varying combinations, then centrifuged to separate pellet and supernatant fractions, which were analysed by polyacrylamide gel electrophoresis to determine how much of the Hsc70 remained in the supernatant or pelleted with the fibres under different conditions.
    Collection period:

    FromTo
    2021UNSPECIFIED
    Temporal coverage:

    FromTo
    2022UNSPECIFIED
    Statement on legal, ethical, and access issues:

    Not applicable
    Depositing User: Helen Saibil
    Date Deposited: 23 Jan 2024 16:03
    Last Modified: 23 Jan 2024 16:06
    Publisher: Birkbeck College, University of London

    Export / Share Citation

    Cite as: Saibil, Helen and MONISTROL, Jim and Johnston, Erin (2023): SDS gels of amyloid fibre-chaperone complexes. Birkbeck College, University of London. doi: https://doi.org/10.18743/DATA.00000326

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