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    Gels of amyloid-chaperone binding assays: control with isolated J-domain of DNAJB1

    Gels of amyloid-chaperone binding assays: control with isolated J-domain of DNAJB1

    Cite as: Dang, Thi Lieu and Bukau, Bernd and Monistrol, Jim and Johnston, Erin and Saibil, Helen R (2024): Gels of amyloid-chaperone binding assays: control with isolated J-domain of DNAJB1. Birkbeck College, University of London. doi: https://doi.org/10.18743/DATA.00000349

    Description

    gels of alpha synuclein amyloid fibres mixed with DNAJB1, deltaJ-DNAJB1 and isolated J-domain including the G/F linker. The mixtures were separated into pellet and supernatant fractions to assess how much of each chaperone construct bound to the fibres in the pellets.
    3 replicates of each set of gels are provided.

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    Collection Method

    gels of alpha synuclein amyloid fibres mixed with DNAJB1, deltaJ-DNAJB1 and isolated J-domain including the G/F linker. The mixtures were separated into pellet and supernatant fractions to assess how much of each chaperone construct bound to the fibres in the pellets.

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    Metadata

    Dataset Title:

    Gels of amyloid-chaperone binding assays: control with isolated J-domain of DNAJB1
    Creators:

    Dang, Thi Lieu and Bukau, Bernd and Monistrol, Jim and Johnston, Erin and Saibil, Helen R
    School/Department:

    Birkbeck Schools and Research Centres > School of Science > Biological Sciences
    Keywords:

    J-domain protein, alpha synuclein amyloid fibres, chaperone-substrate binding
    Data collection method:

    gels of alpha synuclein amyloid fibres mixed with DNAJB1, deltaJ-DNAJB1 and isolated J-domain including the G/F linker. The mixtures were separated into pellet and supernatant fractions to assess how much of each chaperone construct bound to the fibres in the pellets.
    Collection period:

    FromTo
    2024UNSPECIFIED
    Temporal coverage:

    FromTo
    2024UNSPECIFIED
    Statement on legal, ethical, and access issues:

    not applicable
    Depositing User: Helen Saibil
    Date Deposited: 19 Feb 2025 16:58
    Last Modified: 19 Feb 2025 16:58
    Publisher: Birkbeck College, University of London

    Export / Share Citation

    Cite as: Dang, Thi Lieu and Bukau, Bernd and Monistrol, Jim and Johnston, Erin and Saibil, Helen R (2024): Gels of amyloid-chaperone binding assays: control with isolated J-domain of DNAJB1. Birkbeck College, University of London. doi: https://doi.org/10.18743/DATA.00000349

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